Enzyme X is a highly pigmented protein that imparts the characteristic color to certain blue-green algae....

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Chemistry

Enzyme X is a highly pigmented protein that imparts thecharacteristic color to certain blue-green algae. It alsofacilitates a reaction necessary to the survival of this species;we can follow the kinetics of this reaction by measuring theconversion of Substance X to Substance Y at various times duringpurification.
1. Given a pure preparation of these algae, and the requiredsupplies and equipment, devise and outline an empirical procedurefor purifying Enzyme X.
2. What is a good indication of purity in your preparation?
The outline of steps to purify Enzyme X should include detail ofwhat the technique is, why that technique is needed, and what theresult will be. The reader needs to have enough information so asto be able to do the experiment. Treat the assignment as one thatdemonstrates your knowledge of biochemical techniques in proteinpurification.
As for the length of this assignment, you are describing aprotocol; therefore, you need to provide the necessary informationfor the reader to be able to know how, why, and what, as well asthe potential outcomes of the steps and the end result.

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4.1 Ratings (839 Votes)

Do a quantitative assay of the supernatant and the precipitate to see which one has the highest specific activity Keep the one that has the highest The specific activity is the ratio of biochemical activity to the mg of total protein present In this case the conversion of Substance X to Substance Y is the activity that we are See Answer

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