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Enzyme Used Lysozyme Thermal Denaturation Temp (Celsius) Abs (325 nm) 40 0.106 50 0.086 60 0.109 65 0.161 70 0.409 75 2.06 80 2.173 90 0.821 Urea Denaturation M of urea Abs (325 nm) 0 0.464 1 0.07 2 0.056 4 0.05 6 0.035 8 0.028 Please help me Calculate fraction denatured...

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Verified Solution

Question

Chemistry

Enzyme UsedLysozyme
Thermal Denaturation
Temp (Celsius)Abs (325 nm)
400.106
500.086
600.109
650.161
700.409
752.06
802.173
900.821
Urea Denaturation
Mof ureaAbs (325 nm)
00.464
10.07
20.056
40.05
60.035
80.028

Please help me Calculate fraction denatured and equilibriumconstant at each temperature and plot ln K vs T-1 graph, onceplotted Find ΔH, ΔS and ΔG(T=75) (mention any assumptions you made)and What is the Tm for each protein

Answer & Explanation Solved by verified expert
3.8 Ratings (666 Votes)
SolutionDelta G RT ln KFrom data of at 65    See Answer
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