Ectoine is a compatible solute produced by halophilic bacterium as an osmoregulatory compound in a harsh...

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Biology

Ectoine is a compatible solute produced by halophilic bacterium asan osmoregulatory compound in a harsh environment. Its attractiveproperties such as UV-radiation reduction and anti-inflammation. Torecover intracellular ectoine, cell disruption needs to beconducted. Provide one mechanical disruption method and onechemical disruption method that is used to recover intracellularectoine, covering the aspects of working principles, advantages anddisadvantages. Recommend a relatively more suitable method torecover the intracellular ectoine at industrial scale withproviding scientific justifications.

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In the microorganisms ectoine and 5hydroxyectoine are not only accumulated as excellent stress protectants but can also serve as valuable nutrients for cell growth Similar gene clusters involving in ectoine catabolism were disclosed in strain Sinorhizobium meliloti21 Ruegeria pomeroyi DSS3 22 and H elongata DSM 2581T In H elongata DSM 2581T the gene cluster comprising of doeA ectoine hydrolase doeB NaacetylL24diaminobutyric acid deacetylase doeC aspartatesemialdehyde dehydrogenase and doeDdiaminobutyric acid transaminase was verified by inframe deletion Recently more detailed catabolic pathway as well as its regulatory system was identified in strain R pomeroyi DSS3 These genetic circuits suggest avenues for the genetic controlling of ectoine accumulation and are valuable for ectoine production Since hyperosmolarity pressure is required for compatible solutes accumulation fermentation medium with high salinity is generally used for their enrichments and raise big challenges for the fermenter antirust and wastewater treatment Therefore lowsalinity medium is desirable for largescale ectoine production as well as for some other compatible solutes H hydrothermalis Y2 was isolated from an artificial alkaline environment of pulp mill wastewater As a halotolerant extremophile the strain grows well in a broad range salinity that from 0 to 180 g L1 NaCl 26 As we previously observed four NaH antiporters work in a labor division way to deal with saline and alkaline environments in which NhaD2 and Mrp play a notable physiological role in pH and osmotic homeostasis In the present study based on a double mutant that lacking doeA and ectD genes Mrp and or NhaD2 were inframe deleted and their effluence to the ectoine productivity was investigated recovery of intracellular ectoine using the PPGsalt ABS In this study ectoine exhibited a saltrich bottom phase preference in the PPGsalt ABS because of the hydrophilic surface properties of ectoine The effects of concentrations of PPG 425 and salts crude load pH and concentrations of neutral salts NaCl and KCl on the recovery of ectoine were investigated Optimum recovery of intracellular ectoine was obtained See Answer

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