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DNA replication requires many enzymes to accomplish the process successfully. This results in a replication process...

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Biology

DNA replication requires many enzymes to accomplish the processsuccessfully. This results in a replication process that is knownto be bi-directional, semi-conservative and semi-discontinuous. Youdiscover a new prokaryotic organism that lacks the gene coding foran DNA polymerase I enzyme, and therefore this enzyme is not madein this species. Additionally, the DNA polymerase III enzyme fromthis organism lacks any 3’ to 5’ nuclease activity but has 5’ to 3’nuclease activity. Based on what you know about DNA replication andthe functions of DNA polymerase I and DNA polymerase III, discussthe effect of these changes on DNA replication in this organism.Please explain your answer in full.

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3.7 Ratings (307 Votes)
Answer DNA polymerase I is the enzyme which has 5 to 3 polymerase activity which it uses to synthesise DNA by using DNA as template This enzyme also has 3 to 5 exonuclease activity which it uses for proofreading if the wrong nucleotide is added It moves back one nucleotide and by 3 exonuclease activity it removes the wrong nucleotide and replaces it with correct nucleotide The DNA polymerase I also has 5 to 3 exonuclease activity    See Answer
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