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DNA polymerases in all organisms add only 5’ nucleotides to the 3’ end of a growing...

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Biology

DNA polymerases in all organisms add only 5’ nucleotides to the3’ end of a growing DNA strand, never to the 5’end. One possiblereason for this is the fact that most DNA polymerases have aproofreading function that would not be possible if DNA synthesisoccurred in the 3’-5’ direction.

a. Sketch the reaction that DNA polymerase would have tocatalyze if DNA synthesis occurred in the 3’-5’ direction.

b. Consider the information in your sketch and speculate as towhy proofreading would be problematic.

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