DNA Polymerase can distinguish between dNTPs and rNTPs because of discriminator amino acids in the enzyme's...

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Biology

DNA Polymerase can distinguish between dNTPs and rNTPs becauseof discriminator amino acids in the enzyme's nucleotide-bindingpocket. These amino acids occupy the space where the 2'OH group ofan incoming rNTP would need to reside in order to properly positionthe substrates for catalysis. These discriminator amino acidsusually have large R groups, which sterically exclude the ribose2'OH. If you experimentally mutate/change the discriminator aminoacids to glycines, predict the effect that this change might haveon DNA polymerase.

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DNA synthesis is catalysed by enzyme DNA polymerase Thestructure of DNA polymerse resembles a palm whose catalytic siteis made up of sheet thatbinds to divalent metal ions Mg 2 or zn2 DNA polymerases use the same active site to add allthe four dNTPs At the same time DNA polymerase is also able todistinguish See Answer

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