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Discuss the chemical reactions that occurred between glucose, fructose, xylose and sucrose and the 4 reagents(Benedict,...

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Chemistry

Discuss the chemical reactions that occurred between glucose,fructose, xylose and sucrose and the 4 reagents(Benedict, Bial,Seilwanoff and Molisch) which have resulted in color changes.Describe how it occurs in full using the chemical makeup of thereagents.

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3.9 Ratings (662 Votes)

Bial’s reagent

Test for pentoses.components include orcinol,HCl,ferric chloride.Pentoses react to form furfural ,which gives colored substance.pentoses gives blue/green

Positive for pentose sugar -xylose

Benedict’s

-Free aldehyde or keto group in reducing sugars reduce cupric hydroxide in alkaline medium to red colored cuprous oxide.All monosaccarides are reducing .

Seilwanoff

Color reaction specific for ketoses.

Ketoses+conc HCl undergoes dehydration to yield 5-hydroxymethylfurfural derivatives that form deep red color complex with resorcinol. Faster rxn than aldoses

Molisch

All pentose sugar and hexoses are dehydrated by conc. Sulphuric acid to form furfural or hydroxymethylfurfural, respectively. These products condense with α-naphthol to form purple colored condensation product.

glucose

Negative -hexose

positive

negative

positive

fructose

Negative -hexose

positive

positive

positive

xylose

positive

Positive

negative

positive

sucrose

Negative-dimer of hexose

Negative-disaccharides

positive

positive
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