Discuss capillary (zone) electrophoresis, micellar electrokinetic chromatography (MEKC) and capillary electrochromatography. Describe similarities and differences in working principle,...

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Chemistry

Discuss capillary (zone) electrophoresis, micellarelectrokinetic chromatography (MEKC) and capillaryelectrochromatography. Describe similarities and differences inworking principle, injection, and separation. Discuss advantagesand disadvantages of each of these techniques. Which compoundswould you separate with each of these techniques?

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Capillary electrophoresis is an analytical technique that separates ions based on their electrophoretic mobility with the use of an applied voltage The electrophoretic mobility is dependent upon the charge of the molecule the viscosity and the atoms radius The rate at which the particle moves is directly proportional to the applied electric fieldthe greater the field strength the faster the mobility Neutral species are not affected only ions move with the electric field If two ions are the same size the one with greater charge will move the fastest For ions of the same charge the smaller particle has less friction and overall faster migration rate Capillary electrophoresis is used most predominately because it gives faster results and provides high resolution separation It is a useful technique because there is a See Answer

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