1. Directions: Identify the restriction sites foreach of the examples given. Show the cuts, mention if they aresticky ends or blunt, 5’ overhangs or 3 ‘overhangs and the numberof DNA fragments produced and the number of base pairs in each(count the top row).

           Remember: Analyze in the 5’à 3’ direction

1. HindII--- 5' GTC ↓GAC 3'

5' ACGACGTAGTCGACTTATTATGTCGACCCGCCGCGTGTCGACCATCA 3'

3'TGCTGCATCAGCTGAATAATACAGCTGGGCGGCGCACAGCTGGTAGT 5'

Number of pieces of DNA _______

2. EcoRI ---5' G ↓AATTC 3'

5' ACG ACGTATTAGAATTCTTATCCGCCGCCGGAATTCT CATCA 3'

3' TGCTGCATAATCTTAAGAATAGGCGGCGGCCTTAAGAGTAGT 5'

Number of pieces of DNA _______

3. HaeIII--- 5' CC ↓ GG 3'

5' ACGCCGGCCGTATTAT CCGGATCCGCCGCCGGCTGTCCCGGATCA 3'

3'TGCGGCCGGCATAATAGGCCTAGGCGGCGGCCGACAGGGCCTAGT 5'

Number of pieces of DNA _______

4. BamI ---5' CCTAG ↓G 3'

5' ACGCCTAGGACGTATTATCCTAGGTATCCGCCGCCGT CATCA 3'

3'TGCGGATCCTGCATAATAGGATCCATAGGCGGCGGCAGTAGT 5'

Number of pieces of DNA _______

5. HindII--- 5' GTC ↓- GAC 3' andHaeIII --- 5' CC ↓ GG3'

5'ACGGTCGACACGTATTATTAGTCGACTCCGCCGCCGCCGGTCATCA 3'

3'TGCCAGCTGTGCATAATAATCAGCTGAGGCGGCGGCGGCCAGTAGT 5'

Number of pieces of DNA _______

6.  HindII --- 5' GTC ↓ GAC3', HaeIII --- 5' CC ↓GG 3' and BamI --- 5'CCTAG ↓ G 3'

5' ACGCCGGACGTACCTAGGTTTAGTCGACTCCGCCG CCCCTAGGGTCATCA 3'

3'TGCGGCCTGCATGGATCCAAATCAGCTGAGGCGGCGGGGATCCCAGTAGT 5'

Number of pieces of DNA _______