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Devise a scheme of cutting out YGOI insert from the donor plasmid and placing it into...

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Question

Biology

  1. Devise a scheme of cutting out YGOI insert from the donorplasmid and placing it into the recipient plasmid.
  2. How would you separate the transformed cells (those that havepicked up the recombinant plasmid) from non-transformed cells?

Answer & Explanation Solved by verified expert
3.7 Ratings (686 Votes)
YGOI stands for your gene of interest This technique is applicable when a gene of interest is used to multiply to recover desired product For this process a gene of interest from a specific donor a cloning vector that is used to to multiply into a host cell after its introduction and a specific host are required Lets gets stepwise process 1 The gene of interest is separated from a particular donor or plasmid mentioned here For the separation of the gene the enzyme    See Answer
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