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Determine the Vmax and the Km of the enzyme in the absence and presence of each...

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Chemistry

Determine the Vmax and the Km of the enzyme in the absence andpresence of each inhibitor. Calculate the KI for each inhibitor.Are the inhibitors all the same? Which is the best inhibitor andwhy? What is the catalytic efficiency and turnover number of theenzyme knowing that during the experiments its concentration was 2nM? What happens to the catalytic efficiency and turnover number ofthe enzyme in the presence of each of the inhibitor?

The following velocity data were obtained for an enzymaticreaction in the absence and presence of three different inhibitors(A, B, and C):

Initial velocity (v) Initial velocity (v) Initial velocity (v)Initial Velocity (v)

[S] Control (no inhibitor) (+A at 6μM) (+B at 30μM) (+C at4mM)

(mM) (nM/min) (nM/min) (nM/min) (nM/min)

0.200 16.67 6.25 5.56 10.00

0.250 20.00 7.69 6.67 11.11

0.333 24.98 10.00 8.33 12.50

0.500 33.33 14.29 11.11 14.29

1.00 50.00 25.00 16.67 16.67

2.00 66.67 40.00 22.22 18.18

2.50 71.40 45.45 23.81 18.52

3.33 76.92 52.63 25.64 18.87

4.00 80.00 57.14 26.67 19.00

5.00 83.33 62.50 27.77 19.23

Answer & Explanation Solved by verified expert
4.2 Ratings (826 Votes)
Plot 1S and 1Vo for all the four data setsxintercept 1Kmyintercept 1Vmaxenzyme turnover Kcat VmaxEnzymeGraphs are shown below1 without    See Answer
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