Describe the process of gene regulation using the diphtheria toxin gene. More correct, relevant detail is...

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Biology

Describe the process of gene regulation using the diphtheriatoxin gene. More correct, relevant detail is better.

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Transcription of the bacteriophageborne diphtheria toxin gene tox is negatively regulated in response to intracellular Fe2 concentration by the chromosomally encoded diphtheria toxin repressor DtxR Due to a scarcity of tools genetic analysis of Corynebacterium diphtheriae has primarily relied on analysis of chemically induced and spontaneously occurring mutants and on the results of experiments with C diphtheriae genes cloned in Escherichia coli or analyzed in vitro We modified a Tn5based mutagenesis technique for use with C diphtheriae and we used it to construct the first transposon insertion libraries in the chromosome of this grampositive pathogen We isolated two insertions that affected expression of DtxR one 121 bp upstream of dtxR and the other within an essential region of the dtxR coding See Answer

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