-Describe the process of gel electrophoresis. How would the distance that each DNA fragment travelled on...

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Biology

-Describe the process of gel electrophoresis. How would thedistance that each DNA fragment travelled on your gel change if youwere to make the gel using a higher percentage of agarose (forexample 3%)? Explain
-Describe the steps involved in a polymerase chain reaction(PCR), including the changes in temperature that are performed bythe thermocycler and the purpose of each temperature change.

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Question 1Gel electrophoresis is a technique which is used for theseparation of DNA fragments macromolecules and proteins dependingon their size and charge Agarose gel electrophoresis is the methodused for the separation of the DNA fragments in the sample based onthe size All the DNA molecules does have same charge per masshence the separation of DNA in the gel will be based on theirmolecular weight As the name implies the gel electrophoresisconsists of a gel and for the separation of DNA agarose gel isused Agarose is a polysaccharide and for making the gel theagarose powder is mixed in buffer heat it to completely dissolvethe agarose After cooling at room temperature agarose forms asolid gel in which agarose molecules are held together by hydrogenbonds and the gel will have tiny pores with definite size in itThe See Answer

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