Describe the procedure you would follow to determine the concentration of Vitamin C in a sample...

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Chemistry

Describe the procedure you would follow to determine theconcentration of Vitamin C in a sample if the volume of DCPIPrequired to reach the endpoint was more than the 25-mL flask couldhold. Note the titration must still be carried out in the flask. Besure to include any calculations you would need to perform.

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The assay The principle of this method is a titration with dichlorophenolindophenol or phenolindo26dichlorophenol also known as DCPIP Ascorbic acid reacts with DCPIP changing the colour from blue to colourless They react in a 11 fashion so if a known quantity of DCPIP solution reacts with the plant tissue extract the quantity of DCPIP used gives a direct measure of the quantity of ascorbic acid present Grind up a measured mass of fruit or vegetable in a known volume of distilled water Try a ratio of perhaps 1 g fruit to 5 cm3 water See Answer

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