Describe the principles of immunocytochemistry/immunohistochemistry and how the technique can be usefully employed in the diagnosis and...

70.2K

Verified Solution

Question

Chemistry

Describe the principles ofimmunocytochemistry/immunohistochemistry and how the technique canbe usefully employed in the diagnosis and classification oftumours

Answer & Explanation Solved by verified expert

3.6 Ratings (608 Votes)

Principles of immunohistochemistry The principle of IHC has existed since the 1930s but it was not until 1941 that the first IHC study was reportedCoons and his colleagues used Fluorescein isothiocyanate FITClabeled antibodies with a fluorescent dye to localize pneumococcal antigens in infected tissues With the expansion and development of IHC technique enzyme labels have been introduced such as peroxidase89 and alkaline phosphatase Colloidal gold label has also been discovered and used to identify immunohistochemical reactions at both light and electron microscopy levels Other labels include radioactive elements and the immunoreaction can be visualized by autoradiography The aim of IHC is to perform most IHC staining by causing See Answer

Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:

Unlimited Question Access with detailed Answers
Zin AI - 3 Million Words
10 Dall-E 3 Images
20 Plot Generations
Conversation with Dialogue Memory
No Ads, Ever!
Access to Our Best AI Platform: Flex AI - Your personal assistant for all your inquiries!

Become a Member