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Describe the principles behind and the applications of the following: a) Reverse transcriptase-PCR b) Cloning DNA into...

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Biology

Describe the principles behind and the applications of thefollowing:

a) Reverse transcriptase-PCR b) Cloning DNA into a plasmidvector

c) SDS-PAGE

d) Restriction mapping

e) Sanger Sequencing of DNA

Note

Detail explanation is required for each .

Answer & Explanation Solved by verified expert
3.8 Ratings (445 Votes)
a Reverse transcriptase polymerase chain reaction RT PCR is most reliable molecular biology method for detection of RNA expression In this method The RNA is converted to complentary DNA cDNA using reverse transcriptase enzyme RTPCR helps to determine the RNA expression qualitatively In RTPCR the cloned genes expression is studied by producing cDNA from RNA using reverse transcriptase and further the DNA is amplified into    See Answer
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