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Describe the following terms in reference to flow cytometry analysis of immune cells: hydrodynamic focusing, forward...

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Biology

Describe the following terms in reference to flow cytometryanalysis of immune cells: hydrodynamic focusing, forward lightscatter, side light scatter, 2 dimensional scatter plot, threshold,bandpass filter, fluorophore, and CD marker?

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Hydrodynamic Focusing in flow cytometry Hydrodynamic focusing is a technique that enables users of flow cytometry cells to gauge the size of particles in a flow channel whether they be blood cells viruses or bacteria As particles enter the chamber they will typically pass through a laser beam which causes a temporary disruption in the optics Forward light scatter in flow cytometry One detector measures scatter along the path of the laser    See Answer
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