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Describe the chromatofocusing process including a) how separation is enacted b) what pH conditions you need...

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Chemistry

Describe the chromatofocusing process including a) how separationis enacted b) what pH conditions you need and why c) someapplications of this technology d) contrast this with IMAC e) whatapplications might work with IMAC that may not work with ionexchange or chromatofocusing and why? Please list references whenanswering

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4.1 Ratings (651 Votes)
a The technique is used for improperly purified samples This chromatography technique is used for purification of proteins The medium used for the separation of the proteins is buffered at suitable pH above highest pH required The elutant is also buffer as per the lowest pH required When the    See Answer
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