Describe the basic process to clone a cDNA vs. genomic library using PCR technique

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Biology

Describe the basic process to clone a cDNA vs. genomic libraryusing PCR technique

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Meaning of cDNA Library A cDNA library is defined as a collection of cDNA fragments each of which has been cloned into a separate vector molecule Principle of cDNA Library In the case of cDNA libraries we produce DNA copies of the RNA sequences usually the mRNA of an organism and clone them It is called a cDNA library because all the DNA in dis library is complementary to mRNA and is produced by the reverse transcription of the latter Much of eukaryotic DNA consists of repetitive sequences that are not transcribed into mRNA and the sequences are not represented in a cDNA library It must be noted that prokaryotes and lower eukaryotes do not contain introns and preparation of cDNA is generally unnecessary for these organisms Hence cDNA libraries are produced only from higher eukaryotes Vectors used in the Construction of cDNA Library Both the bacterial and bacteriophage DNA are used as vectors in the construction of the cDNA library It is sometimes the case that we wish to clone a particular cDNA for which we already have some sequence data but wif a particular emphasis on the integrity of the 5 or 3 ends RACE techniques Rapid Amplification of cDNA Ends are available for dis The RACE methods See Answer

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