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Describe Surface-Enhanced Laser Desorption Ionization - Time of Flight (SELDI-TOF) mass spectrometry as an approach for...

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Biology

Describe Surface-Enhanced Laser Desorption Ionization - Time ofFlight (SELDI-TOF) mass spectrometry as an approach for the rapiddetection and quantification of proteins.

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Surfaceenhanced laser desorptionionization SELDI is a combination of solidphase chromatography and timeofflight mass spectrometry TOFMS The binding of various protein classes is due the different coatings on the chips ProteinChip arrays are available with different chromatographic properties including reverse phase H50 hydrophobic hydrophilic weak ion exchange CM10WCX2 and immobilizedmetal affinity surfaces IMAC3IMAC30 or they are preactivated for the coupling of ligands To reduce the sample complexity and to enrich target molecules depending on the chip surface each analysis involves an    See Answer
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