Describe, in detail, how to make 1 liter of LB Kan (100 g mlfinal co...

Describe, in detail, how to make 1 liter of LB + Kan (100 Î¼g/ml final concentration)...

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Biology

Describe, in detail, how to make 1 liter of LB + Kan (100 Î¼g/mlfinal concentration) + Amp (50 Î¼g /ml final concentration) liquidmedia. [Include things like how many grams of each component thatyou use, how much antibiotic (in ml) to add (stock solutions â€“ 100mg/ml ampicillin, 25 mg/ml kanamycin), and in what type ofcontainer you perform the sterilization step.] Show yourcalculations.

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3.9 Ratings (367 Votes)

For 1 liter of LB media Preparation Lb stands for Luria Bertani List of components Tryptone10gm Yeast extractbeef extract 5gm Nacl 10gm The final volume of distilled water 1000ml1 liter Add all the components in the 800 ml of distilled water in reagent bottle or conical flask and finally make up the volume to See Answer

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