Logo
Home Study Material Contact Search
AI Chat FREE
  1. sciences
  2. biology
  3. describe, in detail, how to make 1 liter of lb + k

Describe, in detail, how to make 1 liter of LB + Kan (100 μg/ml final concentration)...

80.2K

Verified Solution

Question

Biology

Describe, in detail, how to make 1 liter of LB + Kan (100 μg/mlfinal concentration) + Amp (50 μg /ml final concentration) liquidmedia. [Include things like how many grams of each component thatyou use, how much antibiotic (in ml) to add (stock solutions – 100mg/ml ampicillin, 25 mg/ml kanamycin), and in what type ofcontainer you perform the sterilization step.] Show yourcalculations.

Answer & Explanation Solved by verified expert
3.9 Ratings (367 Votes)
For 1 liter of LB media Preparation Lb stands for Luria Bertani List of components Tryptone10gm Yeast extractbeef extract 5gm Nacl 10gm The final volume of distilled water 1000ml1 liter Add all the components in the 800 ml of distilled water in reagent bottle or conical flask and finally make up the volume to    See Answer
Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:
  • Unlimited Question Access with detailed Answers
  • Zin AI - 3 Million Words
  • 10 Dall-E 3 Images
  • 20 Plot Generations
  • Conversation with Dialogue Memory
  • No Ads, Ever!
  • Access to Our Best AI Platform: Zin AI - Your personal assistant for all your inquiries!
Become a Member

Other questions asked by students

Q

What are the equations of the asymptotes of the graph of f x 1 11...

Algebra
Q

  It is required to select a ‘chemical feed pump’ to transport corrosive chemicals such as...

Mechanical Engineering
Q

SimUText 2022 2023 File Edit Go Tools Help CONTENTS Section 4 An Extended Exploration The...

Biology
Q

2016 97 The temperature inside a refrigerator is t C and the room temperature is...

Physics
Q

Round your answer to the nearest dollar Make a 5 offer on a Ford Escort...

Basic Math
Q

Jim owns a vehicle. Select all of the mutually exclusive events from the following events.Jim's...

Basic Math
Q

A microscope allows a scientist to see a circular region that is 1.29 millimeters in...

Basic Math
Q

You inberit 10 000 Stipulation that for the first Year the money in tuo Stocks...

Algebra
Q

Southwest Milling Co purchased a front-end loader to move stacks of...

Accounting
Q

Carver Company uses a plant-wide overhead rate based on direct labour cost. Suppose that during...

Accounting
Q

George and Meredith who are married, have a regular tax liability of $22,783 based on...

Accounting
Q

0 /14 Date Transaction 1/1/2017 Reeves Corporation issued 100,000 shares of $1.00 par common stock...

Accounting