Describe how you can use PCR to obtain a gene fragment X that
can be cloned...
90.2K
Verified Solution
Link Copied!
Question
Biology
Describe how you can use PCR to obtain a gene fragment X thatcan be cloned into a plasmid vector for expression of the recombinant protein. Includein your description how the PCR reaction will be performed, how the amplicon will beanalyzed, the use of restriction enzymes, how cloning will be achieved, how you willknow that you have a recombinant containing the insert gene fragment X and how you willdetect the recombinant protein.
Answer & Explanation
Solved by verified expert
4.4 Ratings (945 Votes)
We will clone the gene in the following steps1 amplification of gene if interstFirst we will amplify the gene of interst using specific primersof the gene of interst We will design the priners in such a waythat they must contain the restriction enzyme site present in theplasmid in which we have to
See Answer
Get Answers to Unlimited Questions
Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!
Membership Benefits:
Unlimited Question Access with detailed Answers
Zin AI - 3 Million Words
10 Dall-E 3 Images
20 Plot Generations
Conversation with Dialogue Memory
No Ads, Ever!
Access to Our Best AI Platform: Zin AI - Your personal assistant for all your inquiries!
