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Describe how the solvents worked as the mobile phase of the liquid chromatography experiment. Why was...

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Chemistry

Describe how the solvents worked as the mobile phase of theliquid chromatography experiment. Why was it necessary to usedifferent concentrations of aqueous isopropanol in thestep-gradient separation?

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In liquid chromatography a highly polar or highly nonpolar liquid is kept as stationary phase The mixture of components are allowed to be dissolved in it Now when a less polar solvent more polar for 2nd type of stationary phase termed as mobile phase is flowed then the less polar more polar for 2nd type components of the mixture gets dissolved in it and goes with it Less is the polarity of the    See Answer
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