Describe how the solvents worked as the mobile phase of the liquid chromatography experiment. Why was...

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Chemistry

Describe how the solvents worked as the mobile phase of theliquid chromatography experiment. Why was it necessary to usedifferent concentrations of aqueous isopropanol in thestep-gradient separation?

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Liquid chromatography is a technique used to separate a sample into its individual parts This separation occurs based on the interactions of the sample with the mobile and stationary phases Because there are many stationarymobile phase combinations that can be employed when separating a mixture there are several different types of chromatography that are classified based on the physical states of those phases Liquidsolid column chromatography the most popular chromatography technique and the one discussed here features a liquid mobile phase which slowly filters down through the solid stationary phase bringing the separated components with it Components within a mixture are separated in a column based on each components affinity for the mobile phase So if the components are of different polarities and a mobile phase of a distinct polarity is passed through the column one component will migrate through the column faster See Answer

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