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Describe a protocol for isolating mitochondria from rat liver and for estimating the purity of the...

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Biology

Describe a protocol for isolating mitochondria from rat liverand for estimating the purity of the preparation.

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Preparation of isolation buffer Isolation buffer consists of 4099 grams of 225mM Mannitol 3567 grams 75mM sucrose 00744 grams of 02mM EDTA being dissolved in 1000 ml of water Isolation protocol 1 Sacrifice the rat and dissect the liver out and put it in isolation medium icecold 2 Use 15 g of the tissue for the    See Answer
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