Describe 3 sources of human error associated with the addition of
CPA’s and the freezing process...
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Biology
Describe 3 sources of human error associated with the addition ofCPA’s and the freezing process and explain how the errors willaffect the cells
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Answer Understanding the role of water and the need to adequately remove it from cells or abrogate its ability to form ice crystals which damage the cell membrane is critical to successful cryopreservation When cells are frozen in aqueous suspension often they are destroyed However in the 1940s Polge and others discovered the cryoprotective properties of glycerol Since then several chemicals generically called cryoprotectant agents CPAs have been identied The mechanism of action of CPAs is complex and is not fully understood However
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