Custom made primers are shipped as DNA powders. A student received a tube containing 19.5 nmoles...

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Biology

Custom made primers are shipped as DNA powders. A studentreceived a tube containing 19.5 nmoles of primer. To make 75 ÂµMprimer solution, she needs to add ___ Âµl water to the tube.

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Ans. Given-

Â Â Â Â Â Â Â Â Â Â Â Moles of DNA = 19.5 mmol = 1.95 x 10^-8 mol

Â Â Â Â Â Â Â Â Â Â Â [Primer] = 75 uM = 7.5 x 10^-5 M

Now,

Â Â Â Â Â Â Â Â Â Â Â Required volume = Moles / Molarity

Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â = (1.95 x 10^-8 mol) / (7.5 x 10^-5 M)Â Â Â Â Â Â Â Â Â Â Â Â ; [1 M = 1 mol/L]

Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â = (1.95 x 10^-8 mol) / (7.5 x 10^-5 mol / L)

Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â = 2.600 x 10^-4 LÂ Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â ; [1 L = 10³ mL]

Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â = 2.600 x 10^-1 mLÂ Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â ; [1 mL = 10³ uL]

Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â = 260.0 uL

Therefore,

To make 75 uM primer solution, she need to add 260.0 uL water to the tube.

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