Cultured liver hepatocytes are convenient for studying bothexocytosis and receptor-mediated, clathrin-dependent endocytosis.As part of an advanced laboratory in cell biology, students arerequired to perform mutation studies on cultured liver hepatocytesto generate data that supports the proposed mechanism ofreceptor-mediated, clathrin-dependent endocytosis. Liverhepatocytes are cultured, mutagenized, and screened for defectivereceptor-mediated endocytosis. In order to distinguish uniquemutants from one another, the students must design a series ofexperiments to distinguish between mutants that have the followingmutations:
a). Cellular adaptor proteins are mutated to benon-functional
b). Clathrin is mutated to be non-functional
c). Dynamin is mutated to be non-functional
