Consider the final reaction in the citric acid cycle, catalyzed by malate dehydrogenase: Malate + NAD+Â Â â‡„ oxaloacetate...

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Chemistry

Consider the final reaction in the citric acid cycle, catalyzedby malate dehydrogenase:
Malate + NAD+Â Â â‡„ oxaloacetate + NADH + H+
Equilibrium concentrations of the reaction components weremeasured as follows:
[malate] = 5.28 mM
[NAD+] = 75 uM
[oxaloacetate] = 14.5 uM
[NADH] = 14.5 uMï‚·
pH = 8.83
a) From these data calculate the equilibrium constant, Keq, forthis reaction.
b) Calculate the standard free-energy change, Î”GÂ°â€™, for thisreaction (hint: remember that
the prime means that the standard [H+] is 1.0 Ã— 10-7 M, not 1M)
c) Calculate the actual free-energy, Î”G, under the followingconditions:
ï‚· [malate] = 6.0 mM
[NAD+] = 100 uM
[oxaloacetate] = 0.001 uM
[NADH] = 0.0001 uM
pH = 8.83

Answer & Explanation Solved by verified expert

3.9 Ratings (689 Votes)

a Malate NAD oxaloacetate NADH HEquilibrium concentrations of the reaction components weremeasured as followsmalate 528 mMNAD 75 uM 0075 mMoxaloacetate 145 uM 00145 mMNADH 145 uM 00145 mMpH 883H See Answer

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