Compare the binding isotherm of normal hemoglobin (at pH 7.4,with 7.8 mM 2,3 BPG) with that of the following variations. Foreach variant, clearly explain why or why not the binding isothermchanges the way it does.
Explain Please!
a) Hemoglobin at pH 6.9, 7.8 mM 2,3 BPG
b) Hemoglobin in which a glutamate residue (at a positionnormally exposed to the solvent) has been substituted for a valineresidue (at pH 7.4, with 7.8 mM 2,3 BPG)
c) Hemoglobin at pH 7.4 with 9.4 mM 2,3 BPG
d) Hemoglobin with a mutation in the beta chain position 94(position FG1) (at pH 7.4, with 7.8 mM 2,3 BPG). In this mutationAsp94 ->His.
