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COMPARE FLUORESCENT MICROSCOPY WITH DARK FIELD MICROSCOPY . WHAT SIMILAR ADVANTAGE DO THEY PROVIDE? AND HOW...

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Medical Sciences

COMPARE FLUORESCENT MICROSCOPY WITH DARK FIELD MICROSCOPY . WHATSIMILAR ADVANTAGE DO THEY PROVIDE? AND HOW DO THEY DIFFER?

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Similar advantages between fluorescence and dark field microscopy Comparison 1 Both microscopes provide images wherein the background is dark and the cellsspecimen are lighted bright Hence it is easier to visualize the specimen clearly Both microscopes produce images in visible light wavelength making it easier to visualize the specimen 2 Live specimens can be visualized by both techniques Live cells can be imaged with fluorescence imaging where the cells can be modified to express the fluorescent protein before imaging 3 High contrast images are created by both microscopes which have higher resolution This is because of the dark background providing better contrast and not obscuring the    See Answer
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