Ch 13: Eukaryotic Transcription Worksheet
This type of RNA Polymerase is responsible for synthesis of tRNA_________
This type of RNA Polymerase is responsible for synthesis of mRNA_________
This type of RNA Polymerase is responsible for synthesis of rRNA_________
This “element†decreases the rate of transcription_____________
This “element†increases the rate of transcription_____________
The locations of these elements maybe found_______________________________.
This protein is required for RNAP II, it cannot bind without it____________
These protein molecules influence the rate of RNAP II initiationof transcription __________ will bind to enhancer sequences to aidRNAP II binding or ________ may bind to silencer sequences.
____________ was responsible for characterizing RNAP II in thisorganism
Eukaryotic RNAP II has a _________ (what charge) jaw-like clampto hold the DNA.
Abortive transcription refers to the fact that the beginning oftranscription in eukaryotes involves ________and ________transcription because unstably bound ribonucleotides are added.
Once a stable RNA:DNA hybrid forms that is approximately ___ribonucleotides long RNAP II will move along the DNA
RNAP II will hold ____ basepairs of DNA within the complex and____ ribonucleotides.
The lid is the location where what happens?
The Pore is where _____________ will enter the complex to beused during transcription.
When the ________sequence arrives in the complex, the complexbecomes unstable. The clamp opens and both the _____ and nascent______ strand are released.
This precursor to the mature and fully processed mRNA is called_______________ ________RNA or a primary transcript.
Other types of post transcriptional modifications includeaddition of this modified nucleotide to the 5’ end, it is called a__________________
What is unique about its attachment to the adjacentribonucleotide?
What are some reasons for its addition?
How long is a typical 3’ poly A tail? Do all eukaryotic mRNAshave a poly A tail?
(True or False) Introns do not make up a significant portion ofthe length of the genomic gene sequence. (In other words, thelength of the intronic DNA is much shorter than the exonic DNA)
Group 1 splicing mechanism involve catalytic _____ moleculescalled _________; these are non protein molecules with enzymaticactivity.
Group 1 splicing mechanisms involve removing introns in primarytranscripts of _____
Introns in mRNAs are removed using a large complex structurecalled a ___________ with the help of binding of small nuclear RNAsbound to proteins called ____________
Small nuclear RNAs have a high concentration of thisribonucleotide_________
A self splicing mechanism involving the formation of a ________structure following snRNA’s complementary binding will cut the RNAand allow exons to be ligated.