Logo
Home Study Material Contact Search
AI Chat FREE
  1. sciences
  2. biology
  3. can you please write about dna extraction from blo

Can you please write about DNA extraction from blood and preparation of blood samples for DNA...

60.1K

Verified Solution

Question

Biology

Can you please write about DNA extraction from blood andpreparation of blood samples for DNA extraction. This is theprotocol:

1.Take 750 μl of blood from each blood sample, and transfer itto new 1.5ml tube;
2. Mix 750 μl of blood with 1ml RBC lysis buffer, respectively(avoid stock contamination by not touching the walls of tube bypipette tip);
3. Centrifuge at 2300 rpm for 5 minutes;
4. Take out the liquid, keep the pellet;
5. Mix 1 ml of RBC lysis buffer with pellet in 15ml centrifugetube;
6. Add PBS up to 14 ml by pipette controller (increase volume andcontrast);
7. Centrifuge at 2200 rpm, at 4°C, for 5 minutes
8. Discard the supernatant, keep the pellet;

9. Add 1 ml of RBC lysis buffer;
10. Add PBS up to 10 ml;
11. Centrifuge at 2200 rpm, at 4°C, for 5 minutes;
12. Discard the supernatant and resuspend the pellet in 1 ml ofTrizol;
13. Store the samples at -20°C

Can you explain the protocol, why are we doing these steps. Alsoif you can say the function of each chemical:

  • RBC lysis buffer
  • PBS
  • Trizol

Thank you in advance. I really really need your help.

Answer & Explanation Solved by verified expert
3.9 Ratings (670 Votes)
Using precipitation chemistry the whole blood DNA isolation acts by precipitating DNA out of lysate via high salt concentration and by addition of an alcohol like ethanol and ispropyl alcohol It seperates DNA from other biomolecules and compounds like lipids and carbohydrates They also seperate DNA from any exogenously added chemical compounds which is present in our blood They can be one step and 2 step lysis methods a Two step lysis method RBCS lysed with an anionic detergent in presence of a DNA stabilizer WBCS lysed gDNA and cellular DNA will be released into the solution by    See Answer
Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:
  • Unlimited Question Access with detailed Answers
  • Zin AI - 3 Million Words
  • 10 Dall-E 3 Images
  • 20 Plot Generations
  • Conversation with Dialogue Memory
  • No Ads, Ever!
  • Access to Our Best AI Platform: Flex AI - Your personal assistant for all your inquiries!
Become a Member

Other questions asked by students