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Can Western blotting be used as a quantitative technique? How could we use Western analysis to...

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Biology

Can Western blotting be used as a quantitative technique? Howcould we use Western analysis to determine the relative change in aprotein's expression levels between two treatments? What would weneed to be able to determine the absolute level of a protein (i.e.ng/mg of total protein)?

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Western Blot is a technique that is used to separate on a polyacrylamide on the basis of their size This technique can also be exploited to quantify the amount of proteins present in a sample However this cannot be done directly and an    See Answer
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