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By gel electrophoresis, how do you know if your protein binded to the column? How would...

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Biology

By gel electrophoresis, how do you know if your protein bindedto the column? How would you modify the purification next time tomake it more efficient?

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3.8 Ratings (556 Votes)
Separation and purification of molecules like nuclei acids lipids or proteins is mainly based on the differences in their physical properties Proteins may vary according to the a Amino acid sequence and chain length b Size in terms of length or mass c Threedimensional structure or spatial arrangement d Net charge determining hydrophilic or    See Answer
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