Biochem Lab: Explain the basis for the stacking gel portion of a polyacrylamide gel. Describe how...

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Chemistry

Biochem Lab: Explain the basis for the stacking gel portion of apolyacrylamide gel. Describe how it works with respect to theloaded protein samples as well as the running buffercomponents.

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Polyacrylamide gel is commonly used in the lab for the separation of proteins based on their molecular weight Its one of the most commonly technique used but a tedious process It separates proteins according to their molecular weight based on their differential rates of migration through a sieving matrix a gel under the influence of an applied electrical field The phenomenon is known as electrophoresis The natively folded proteins neither have net charge nor their molecular radius is molecular weight dependent So this is problem associated with the tertiary structure of the proteins unlike any charged species whose movement can be monitored by the quantity of their net charge molecular radius and magnitude of applied field So in case of proteins Instead their net charge is determined by amino acid See Answer

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