Succinate + NAD+ = Fumerate + NADH + H+
ATP hydrolysis takes place during this reaction,
ATP = ADP + Pi
Given: Steady state reaction, phosphate is
constant at 0.01 M, [NADH]/[succinate] = 0.01 M
Rate = [ADP]
[Pi] = [FUMARATE]
[NADH]Â Â
          Â
ATPÂ Â Â Â Â Â Â Â Â Â Â Â Â
[NAD+] [SUCCINATE]
Hence: NAD+ = FUMARATE, and [NADH] Â Â = 0.01
M
                                                Â
[SUCCINATE]
So, [ADP] [Pi] =
1 (ie, 0.01 divided by 0.01)
       ATP
Rearrange: [ATP]
= 1
               Â
[ADP] [Pi]
