Bacteriophage Lambda phage have the ability to survive either asa lysogen w/in a bacterial host or by lytic growth. Lytic growthinvolves replication of the viral DNA manyfold, assembly ofbacteriophage particles, and cell lysis to release the newinfectious bacteriophage particles. The bacteriophage particles caneach package one entire viral genome of ~50Kb but not much more DNAthan that. Use your knowledge of the regulation of the lambda geneexpression to design a lambda cloning vector. The cloning vectorshould be replicated manyfold, and assemble into infectious viralparticles that are released by lysis of the host bacterial cell.Hint: think about what genes are necessary for lysogenic growth anddispensable for lytic growth. These are the genes that you canreplace with your DNA of interest.
