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Aseptic Technique Introduction Aseptic technique is essential in all microbiological laboratory procedures to ensure that...

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Aseptic Technique Introduction Aseptic technique is essential in all microbiological laboratory procedures to ensure that none of the media or cultures is contaminated with unwanted microorganisms It is extremely important to master this technique to have meaningful results in your exercises and for your independent projects Procedure Inoculate a phenol red broth with Escherichia coli E coli using an inoculating loop as demonstrated by the instructor Make sure to use the tube labeled Eco Flame the loop and mock inoculate a second phenol red broth Put your labeled tubes in the rack up front They will be incubated at 37 C for 2 days at which point your instructor will move the cultures to the fridge until the next lab period

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