Antibody is prepared to the thr repressor protein isolated from a wild-type strain.Â Â Competetive antibody binding assays show...

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Biology

Antibody is prepared to the thr repressor proteinisolated from a wild-type strain.Â Â Competetive antibodybinding assays show that several times more repressor is present inan extract prepared from the derepressed mutant described abovethan in an extract prepared from the wild-typestrain.Â Â What is your interpretation of these data?
The repressor protein requires a pH of 6.1 for optimal bindingto its operator in vitro.Â Â If the pH goes abovepH 8.0, the repressor is unable to bind.Â Â What amino acidside chains are implicated?Â Â Why is binding reduced inalkaline media?
What effect would an increase in pH have on cell transcriptionof the thr operon? Why?
What percentage of the repressor will bind at pH6.3?Â Â More than 50% or less?Â Â Nowcalculate.

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4.5 Ratings (916 Votes)

aDerepressed mutants will allow the increased expression of therepressor Hence for activation of the operon more concentrationof Thr threonine is required Because Thr will bind to therepressor and will not allow it to bind to the operator and henceoperon will be ON However in the derepressed mutant presence See Answer

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