Answer this question in a 1-2 paragraphs The experiment that was performed was size exclusion chromatography. it...

70.2K

Verified Solution

Question

Biology

Answer this question in a 1-2 paragraphs

The experiment that was performed was size exclusionchromatography. it involved:

- sephadex G75 (separates up to 80,000 g/mol)
- buffer: 20mM sodium Phosphate @ ph 7.0
- three compounds that were separated
1.) blue dextron (2,000,000 g/mol) blue
2.) cytochrome C (~12,000 g/mol) orange
3.) potassium chromate (194.2 g/mol) yellow

1 eluded first followed by 2 and then 3 as expected.

••• Come up with a creative, some sort of experiment that canpossibly be performed to consolidate or build on the experimentalready performed. “We are not looking for changes for the currentexperiment, this would be considered minor/ trivial changes andwill lead to only 1 point”.

Answer & Explanation Solved by verified expert
4.0 Ratings (605 Votes)
The compounds can be identified by simply measuring theirabsorbance at maxof each of these compounds maxis the wavelength at which the absorbance of a compound is maximumand is characteristic of    See Answer
Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:
  • Unlimited Question Access with detailed Answers
  • Zin AI - 3 Million Words
  • 10 Dall-E 3 Images
  • 20 Plot Generations
  • Conversation with Dialogue Memory
  • No Ads, Ever!
  • Access to Our Best AI Platform: Flex AI - Your personal assistant for all your inquiries!
Become a Member

Other questions asked by students