Logo
Home Study Material Contact Search
AI Chat FREE
  1. sciences
  2. biology
  3. anion exchange chromatography 1. in the purificati

Anion exchange chromatography 1. In the purification, the pH of the PBS buffer was 6.5, and the...

50.1K

Verified Solution

Question

Biology

Anion exchange chromatography

1. In the purification, the pH of the PBS buffer was6.5, and the two proteins have different isoelectric points(Haemoglobin pI: pI 6.8; Catalase: pI 5.4). What are the chargestates of the two proteins at this pH? (e.g. positively charged,negatively charged or neutral)

2. Based on the charge states of the proteins, whichprotein has a higher affinity to the DEAE-Sepharose resin at pH6.5?

3. Why was the salt concentration of PBS bufferincreased (from 17.5 mM to 175 mM) in order to elute out thecatalase protein?

Answer & Explanation Solved by verified expert
4.4 Ratings (697 Votes)
1Haemoglobin is positively charge at ph 65 Catalase is negatively charged at ph 65 Reason pi is that pH at which there is not net charge on the molecule If the ph of the solution is less than the    See Answer
Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:
  • Unlimited Question Access with detailed Answers
  • Zin AI - 3 Million Words
  • 10 Dall-E 3 Images
  • 20 Plot Generations
  • Conversation with Dialogue Memory
  • No Ads, Ever!
  • Access to Our Best AI Platform: Flex AI - Your personal assistant for all your inquiries!
Become a Member

Other questions asked by students

Finance
1.2K views

2. Please answer with care and attention to all questions. Explain as much as you can...

Physics
1.2K views

why dont we see interference fringes when light shines through a window?

Electrical Engineering
1.2K views

Input pin(s): inputw [1], sysclock [1] Output pin(s): outputq [1] Design a 3-bit parity generator using a...

Basic Math
1.2K views

The time between breakdowns of an alarm system is exponentially distributed with mean 10 days....

Statistics
1.2K views

1 The value of insurance Suppose your classmate Roby is faced with the following decision...

Accounting
1.2K views

EXAMPLE 1.11 A rental company spent $2500 on a new air compressor 7 years ago....

Accounting
1.2K views

Which of the following is not true regarding qualified defined contribution plans? ...

Accounting
1.2K views

HA06 2.20162017: -3120162017120,000120,000: 0006,0006,0006,006,006.57.000: 51,60051,6002,4005,400: 20163,0003,00020173,0004,000. RM12,000.: 8% RM10,800 RM31,200: RM6 RM9...

Accounting
1.2K views

Given the following, what is the total amount of current assets? ...

Accounting
1.2K views

EC2003 2019-20 project fnal.pdf - Foxt Reader Find 20.- Gu FILE HOME COMMENT VIEW FORM...

Accounting
1.2K views

Q1. How worried are clients and stakeholders in day-to- day product improvement? 2. the...

Finance
1.2K views

If a firm has fixed costs of $33,000, a price of $5.00, and a breakeven...