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  3. and transplanted them in mouse 3 which had been th

and transplanted them in mouse 3 which had been thymectomized and lethally irradiated no T...

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Biology

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and transplanted them in mouse 3 which had been thymectomized and lethally irradiated no T cell Use experiments Zinkernagel took bone marrow from mouse 1 and a thymi producing T cells from mouse 1 those T cells will mat ure in thymus and be exposed to self antigens He them challenged the reconstituted mouse with LCM virus and removed spleen cells These splee incubated with LCMV infected target cells cells expressing MHC plus antigenic components of the different MHC haplotypes and the lysis of the target cells was monitored The results of 2 such e using H 2 strain C57BL 6 mouse 1 mice and H 2 mouse 2 strain BALB C mice are shown in the Remember that the TCR has to recognize the MHC antigen complex of the haplotype in which it wa recognize A B Experiment Bone marrow and thymus donor Mouse 1 H 2 x Mouse 2 H 22 Mouse 1 H 22 Mouse 2 H 22 Thymectomized x irradiated recipient Mouse 1 x Mouse 2 H 2bxd Mouse 1 x Mouse 2 H 2bxd Lysis of LCN H 2d All of the cells in these mice suiral antigen

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