An enzyme was found that catalyzes the reaction between students, S and academic success, P (for...

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Chemistry

An enzyme was found that catalyzes the reaction betweenstudents, S and academic success, P (for pass); the enzyme wascalled studyase.
An enzyme assay was run on studyase. 10Î¼g of studyase (molecularweight 20,000D) was placed in a set of test tubes each of whichcontained 1mL of the substrate student at varying concentrationsand the amount of product, academic success (P) was determined.
The following results were determined:
S, (mM)
Velocity, V (Î¼mole/mL/sec)
1
12
2
20
4
29
8
35
12
40
Note 1mM = 1millimole/L = 1Î¼mol/mL
Plot a Lineweaver-Burk plot of 1/V versus 1/S
Determine Vmax and Km for the enzyme studyase.
Calculate Kcat and the catalytic constant (kcat/km) for theenzyme. (find [E]_T as total Î¼moles/mL)
Another enzyme called partyase was analyzed and when 0.010Î¼moles of this enzyme was assayed it was found to have a Vmax ofabout 50 Î¼mol/mL/sec and a Km of 5 mM. Calculate Kcat and thecatalytic constant for this enzyme.
i.Compare the 2 enzymes on the basisof affinity for the binding site and kinetic efficiency.

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4.4 Ratings (823 Votes)

a Lineweaver Burk plotb For the above graph1Vmax Yintercept 0019 Vmax 10019 526 mMs or micromolmLs answerKm Vmax slope 0063 s Km 0063 s x Vmax 0063 s x 526 mMs See Answer

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