An enzyme is discovered that catalyzes the chemical reaction

SAD --> <-- HAPPY

A team of motivated researchers setout to study the enzyme, which they call happyase. Under a constantenzyme concentration, [E] of 2.0 uM, they studied the relationshipbetween substrate concentration [SAD] and reaction velocityV₀. Their data is shown below:

[SAD](uM)               V₀(uMs^-1)      

20                  3.4 x 10³

40                 5.4 x 10³

60                 6.6 x 10³

80                  7.4 x 10³

Can you determineK_cat and K_m from the abovedata?

2. While [E] remains 2.0 um and [SAD] = 12 uM, what would thereaction velocity V₀ be?

3. In a separate happyase experiment using [E] = 1.0 uM, thereaction velocity, V_0, is measured as 3.6 x10³ uMs^-1. What is the [SAD] used in thisexperiment?

4.The researchers working on happyase discovered that thecompound STRESS is a potent inhibitor of happyase. A kinetics studyof happyase in the presenceof         1 nM STRESS,gives the following data:

[SAD](uM)               V₀(uMs^-1)      

20                  1.7 x 10³

40                 2.7 x 10³

60                  3.3 x10^{3              Â} 

80                  3.7 x 10³

Which class of inhibitors does STRESSbelong to?