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  3. an enzymatic reaction was analyzed for rate versus

An enzymatic reaction was analyzed for rate versus substrate concentration. Roughly estimate the Vmax and Km...

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Chemistry

An enzymatic reaction was analyzed for rate versus substrateconcentration. Roughly estimate the Vmax and Km from thesemeasurements. (Hint: Slope = rise/run and remember what Kmrepresents!)

vo(nM/s)          [S] (nM)

30                          100,000

29.5                       10,000

29                          1,000

28                          200                        

25                          150

20                          100

10                          50

5                            20

a. Vmax = 10 nM/s, Km = 20 nM

b. Vmax = 20 nM/s , Km = 50 nM

c. Vmax = 40 nM/s , Km = 150 nM

d. Vmax = 70 nM/s, Km = 250 nM

ANSWER is (c), how?

Answer & Explanation Solved by verified expert
3.6 Ratings (639 Votes)
The MichaelisMenten kineticsvVmaxsKmsHereVmax is the the    See Answer
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