An anion-exchange resin can be used instead of the cation-exchange resin in this experiment to separate...

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Chemistry

An anion-exchange resin can be used instead of thecation-exchange resin in this experiment to separate the threeamino acids in the known mixture. What changes to experimentalprotocol are required to achieve complete resolution? Support yourresponse with an explanation. (HINT: consider pH).
You have been provided a mixture containing four amino acids(Glu, His, Pro and Lys) to be separated by ion-exchangechromatography using a Dowex 50 cation-exchange, prepared in aloading buffer at pH 2.0. The following solutions are madeavailable:
The amino acid mixture in 0.04M citrate buffer at pH 2.0.
0.04M citrate buffer, pH 4.5
0.04M citrate buffer, pH 8.0
0.04M citrate buffer, pH 10.5
Is complete resolution of all amino acids achieved if thebuffers are allowed to elute through the column sequentially fromlowest to highest pH? Identify the order of elution and identifyany amino acids that may come off the column together if completeresolution is not achieved. Support your response with anexplanation.
Assuming the amino acids in the mixture will elute from the columnONLY at a solution pH that is above their respective pI.

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4.3 Ratings (518 Votes)

Solution Important notes In ionexchange chromatograph the molecules bind to oppsitely charged sites in the stationary phase when they passes through the chromatographic column Isoelectric point pI is the pH at which an See Answer

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