Acetylcholinesterase catalyzes the hydrolysis of the neurotransmitter acetylcholine: Acetylcholine + H2O ïƒ acetate + choline The Km...

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Biology

Acetylcholinesterase catalyzes the hydrolysis of theneurotransmitter acetylcholine: Acetylcholine + H2O ïƒ acetate +choline The Km of acetylcholinesterase for its substrateacetylcholine is 9.5x10-5M. In a reaction mixture containing 5nanomoles/mL of acetylcholinesterase and 150Î¼M acetylcholine, avelocity vo=40Î¼mol/mLsec was observed for the acetylcholinesterasereaction.
a. Calculate Vmax for this amount of enzyme
b. Calculate kcat for acetylcholinesterase
c. Calculate the catalytic efficiency (kcat/Km) foracetylcholinesterase
d. Does acetylcholinesterase approach catalytic perfection?
e. What determines the ultimate speed limit of anenzyme-catalyzed reaction? That is, what is it that imposes thephysical limit on catalytic perfection?

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4.0 Ratings (489 Votes)

a We know from MichaelisMenten equation that V Vmax S S Km So Vmax V S Km S 40 molmLsec 95105 M 150 M 150 M We know 1 molmL 0001 M So 40 molmL 400001 M 004 M We know 1 See Answer

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