Logo
Home Study Material Contact Search
AI Chat FREE
  1. sciences
  2. physics
  3. a) you are given a dye with an excitation maximum

A) You are given a dye with an excitation maximum of 358 nm and an emission...

50.1K

Verified Solution

Question

Physics

A) You are given a dye with an excitation maximum of 358 nm andan emission maximum of 461 nm, and your fluorescence microscope hasfluorescence cubes with the filters below. Do you think you willyou be able to see any fluorescence from this dye using any ofthese fluorescence cubes? Explain your answer. B) Does itmake a difference if the barrier filter is a band pass versus along pass? Why?

Filter cube Excitation (exciter) Emission (barrier) DichromaticMirror

WU -> MWU2 330-385 420 400

WB -> MWB2 460-490 520 500

WG -> MWG2 510-550 590 700

Answer & Explanation Solved by verified expert
3.7 Ratings (452 Votes)
A I think we shall be able to see the fluroscence from thegiven dye whose excitation is taking place at 358nm and emission is461nm by using the first dichromatic mirror filter WU MWU2330385 420 400 The fluroscence filters are strategically    See Answer
Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:
  • Unlimited Question Access with detailed Answers
  • Zin AI - 3 Million Words
  • 10 Dall-E 3 Images
  • 20 Plot Generations
  • Conversation with Dialogue Memory
  • No Ads, Ever!
  • Access to Our Best AI Platform: Flex AI - Your personal assistant for all your inquiries!
Become a Member

Other questions asked by students

Psychology
1.2K views

Have you ever thought about personality theory through a biblical worldview? What traits make up your personality?...

General Management
1.2K views

A manufacturing area needs to forecast workhours. This is a slightly different project. The data for...

Geometry
1.2K views

rea Volume 2 Calculate the area and perimeter 10 points HA and the volume and...

Geometry
1.2K views

Graph the function f x 4 2 by moving the key points and the horizontal...

Basic Math
1.2K views

Solve 5ax + 6ax = 7ax+8 for x. Assume a0.O A. x = 2/aO B....

Accounting
1.2K views

If a company uses accrual accounting, then it would record the sale of merchandise on...

Accounting
1.2K views

Quick bites has a restaurant that is opened all day. Fixed costs total $450,000 per...

Accounting
1.2K views

Question 2 Describe THREE (3) importance of the wow stories in customer service for a...

Accounting
1.2K views

Entries for Issuing and Calling Bonds; Gain Mia Breen Corp. produces and sells wind-energy-driven engines....

Accounting
1.2K views

Ence sales, Inc., a merchandise company, reported sales 6,400 units in April at a selling...

Accounting
1.2K views

[97] Gleim #: 6.2.41 Juliet bought and placed in service computer equipment in 2011. She...

Finance
1.2K views

Identify the current capital market risk (US and Canada) by quoting one particular rate (per...