A) You are given a dye with an excitation maximum of 358 nm andan emission maximum of 461 nm, and your fluorescence microscope hasfluorescence cubes with the filters below. Do you think you willyou be able to see any fluorescence from this dye using any ofthese fluorescence cubes? Explain your answer. B) Does itmake a difference if the barrier filter is a band pass versus along pass? Why?
Filter cube Excitation (exciter) Emission (barrier) DichromaticMirror
WU -> MWU2 330-385 420 400
WB -> MWB2 460-490 520 500
WG -> MWG2 510-550 590 700
