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A typical concentration of ampicillin used for bacterial selection in liquid LB is 100 microliter/mL. we...

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Biology

A typical concentration of ampicillin used for bacterialselection in liquid LB is 100 microliter/mL. we prepared a 100mg/mL ampicillin stock solution for your use. if you will only use.5 mL of your prepared dilution to spread on a plate, what is areasonable volume of each dilution to prepare? Do you really needhundreds of milliliters?

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3.6 Ratings (652 Votes)
Ampicillin concentration in stock100 mgmL 05 ml of the solution should contain 100 microlitermL of ampicillin as typical concentration of ampicillin used in bacterial selection is 100 microlitermL Hence 1 ml of the solution should contain 200 microlitermL of ampicillin    See Answer
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